. Rolling circle has basically five steps. 1. DNA will be "nicked". 2. 3' end elongated using "unnicked" DNA as leading strand ; 5' end displaced 3. Displaced dNA is lagging strand and is made double stranded using Okazaki fragments. 4. Replication of both "unnicked" and displaced DNA completed 5. Displaced DNA circularizex
Rolling circle replication describes a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of
DNA or
RNA, such as
plasmids, the
genomes of
bacteriophages, and the
circular RNA genome of
viroids. Some eukaryotic virusesc also replicate their DNA via a rolling circle mechanism.