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Serum protein electrophoresis
Serum protein electrophoresis
(SPEP or SPE) is a laboratory test that examines specific
proteins in the blood
called
globulins
.
Blood
must first be collected, usually into an airtight vial or
syringe
.
Electrophoresis
is a laboratory technique in which the
blood serum
(the fluid portion of the blood after the blood has clotted) is placed into a
gel
, or into liquid in a
capillary
tube, and exposed to an
electric current
to separate the serum protein components into five major fractions by size and electrical charge:
serum albumin
,
alpha-1 globulins
,
alpha-2 globulin
s,
beta globulins
, and
gamma globulins
.
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