U.S. Route 53, or U.S. Highway 53 (U.S. 53), is a north–south U.S. highway that runs for 403 miles (649 km) from La Crosse, Wisconsin to International Falls, Minnesota. It is the primary north–south route in northwestern Wisconsin, serving as a vital link between I-94 at Eau Claire, Wisconsin and the city of Duluth, Minnesota. The entire route from Eau Claire to the city limits of Superior, Wisconsin is a four lane divided highway. The highway's northern terminus is at the Fort Frances-International Falls Bridge in International Falls, Minnesota, at the U.S.-Canadian border. Its southern terminus is in La Crosse, Wisconsin, at U.S. Highway 14.