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Additional
Ultraviolet-visible spectroscopy
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Ultraviolet–visible spectroscopy
Ultraviolet–visible spectroscopy
or
ultraviolet-visible spectrophotometry
(
UV-Vis
or
UV/Vis
) refers to
absorption spectroscopy
or reflectance spectroscopy in the
ultraviolet
-
visible
spectral region. This means it uses light in the visible and adjacent (near-UV and
near-infrared
[NIR]) ranges. The absorption or reflectance in the visible range directly affects the perceived
color of the chemicals
involved. In this region of the
electromagnetic spectrum
,
molecules
undergo
electronic transitions
. This technique is complementary to
fluorescence spectroscopy
, in that
fluorescence
deals with transitions from the
excited state
to the
ground state
, while absorption measures transitions from the ground state to the excited state.
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