The Lord of Annandale was a sub-comital lordship in southern Scotland (Annandale) established by David I of Scotland by 1124 for his follower Robert de Brus. The following were holders of the officers:

• Robert de Brus, 1st Lord of Annandale, 1113 x 1124-1138
• Robert de Brus, 2nd Lord of Annandale, 1138x-1194
• William de Brus, 3rd Lord of Annandale, 1194-1211 x 1212
• In the 13th century, the Carruthers family rose to be the hereditary Stewards of Annandale, under the Bruces
• Robert de Brus, 4th Lord of Annandale, 1211 x 1212-1226 x 1233
• Robert de Brus, 5th Lord of Annandale, 1226 x 1233-1292; resigned the lordship to Annadale on the accession of John Balliol in 1292.
• Robert de Brus, 6th Lord of Annandale, 1292-1295
• John III Comyn, Lord of Badenoch, 1295-1296; Annandale seized and granted to John on Robert's refusal to attend the Scottish host.
• Robert de Brus, 6th Lord of Annandale, 1296-1304
• Robert de Brus, 7th Lord of Annandale (King Robert), 1304-1312
• Thomas Randolph, 8th Lord of Annandale, 1312–32
• Thomas Randolph, 9th Lord of Annandale, 1332
• John Randolph, 10th Lord of Annandale, 1332–46
• Agnes Randolph, 11th Lady of Annandale, 1346-1369
  • m. Patrick Dunbar, 9th Earl of Dunbar
• George de Dunbar, 12th Lord of Annandale, 1369-1401/9 (although under part English control until 1384; conquered by Douglas in 1401 after Dunbar went over to the English; Douglas possession confirmed in 1409)
• Archibald Douglas, 13th Lord of Annandale, 1401/9-24
• Archibald Douglas, 14th Lord of Annandale, 1424-1439
• William Douglas, 15th Lord of Annandale, 1439–40
• Annexed to Crown
• Alexander Stewart, 16th Lord of Annandale, 1455-1485
• John Stewart, 17th Lord of Annandale ?, 1485-1536
• Crown