UNIFI may refer to:

• UNIFI Companies, the company formed by the 2006 merger of Ameritas Acacia Mutual Holding Company and Union Central Life Insurance Company
• UNIFI (trade union), the British trade union that merged into Amicus in 2004
• The University of Florence
• The UNIversal FInancial messaging standard, more formally known as ISO 20022
• Unifi Network, a former division of PricewaterhouseCoopers made up of the Kwasha Lipton Group and its other benefits consulting divisions
• UniFi, a high speed broadband service in Malaysia

UniFi is a triple-play service by Telekom Malaysia, offering of Internet access, VoIP and IPTV which is provided to residential and business customers in Malaysia through an optical fiber core network via FTTH for individual housing units and VDSL2 for multi-story buildings depending on the customer premises.

UNIFI was a trade union representing worked in the finance sector in Britain. The name UNiFI was briefly adopted by the Barclays Group Staff Union in 1999. Later in the year, the union merged with the Banking, Insurance and Finance Union and the NatWest Staff Association, and the new organisation chose the very similar name "UNIFI". In 2004, UNIFI merged with Amicus. The organisation's general secretary was Ed Sweeney, and the national secretary was Rob MacGregor.