LGBT culture or LGBTQI culture is a culture shared by lesbian, gay, bisexual, transgender, questioning and intersex people. It is sometimes referred to as queer culture (indicating people who are queer), while the term gay culture may be used to mean "LGBTQI culture," or to refer specifically to homosexual male culture.

Sexuality and gender identity-based cultures are subcultures and communities composed of people who have shared experiences, backgrounds, or interests due to common sexual or gender identities. Among the first to argue that members of sexual minorities can also constitute cultural minorities were Adolf Brand, Magnus Hirschfeld, and Leontine Sagan in Germany. These pioneers were later followed by the Mattachine Society and the Daughters of Bilitis in the United States.