An
internal standard in
analytical chemistry is a
chemical substance that is added in a constant amount to samples, the blank and
calibration standards in a
chemical analysis. This substance can then be used for calibration by plotting the ratio of the
analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet. The internal standard is a compound that is very similar, but not identical to the chemical species of interest in the samples, as the effects of sample preparation should, relative to the amount of each species, be the same for the signal from the internal standard as for the signal(s) from the species of interest in the ideal case. Adding known quantities of analyte(s) of interest is a distinct technique called
standard addition, which is performed to correct for
matrix effects.