pampa

PAMPA (parallel artificial membrane permeability assay) is a method which determines the permeability of substances from a donor compartment, through a lipid-infused artificial membrane into an acceptor compartment. A multi-well microtitre plate is used for the donor and a membrane/acceptor compartment is placed on top; the whole assembly is commonly referred to as a “sandwich”. At the beginning of the test, the drug is added to the donor compartment, and the acceptor compartment is drug-free. After an incubation period which may include stirring, the sandwich is separated and the amount of drug is measured in each compartment. Mass balance allows calculation of drug that remains in the membrane. To date, PAMPA models have been developed that exhibit a high degree of correlation with permeation across a variety of barriers, including Caco-2 cultures, the gastrointestinal tract, blood–brain barrier and skin.

The Pampas (from Quechua pampa, meaning "plain") are fertile South American lowlands, covering more than , that include the Argentine provinces of Buenos Aires, La Pampa, Santa Fe, Entre Ríos and Córdoba; most of Uruguay; and the southernmost Brazilian State, Rio Grande do Sul. These vast plains are a natural region only interrupted by the low Ventana and Tandil hills near Bahía Blanca and Tandil (Argentina), with a height of and , respectively.